Screening differentially expressed proteins in the serum of patients with systemic lupus erythematosus combined with osteonecrosis of the femoral head by iTRAQ technology

doi:10.3969/j.issn.2095-4344.0583

Abstract

Abstract:

BACKGROUND: Systemic lupus erythematosus (SLE) combined with osteonecrosis of the femoral head (ONFH) is a result of long-term application of steroids that induces death of active ingredients in femoral head. But there is a lack of specific biomarker for early diagnosis.
OBJECTIVE: To screen the systemic markers of SLE with steroid-induced ONFH using isobaric tags for relative and absolute quantitation (iTRAQ) technology.
METHODS: Serum of eight patients with SLE and steroid-induced ONFH was collected and used as the experimental group, and serum of 10 patients with SLE but without steroid-induced ONFH served as the control group. The differentially expressed proteins were screened and identified by iTRAQ technique and two-dimensional liquid chromatography-tandem mass spectrometry.
RESULTS AND CONCLUSION: Totally 10 kinds of differentially expressed proteins were identified. There was significant difference between two groups. In the experimental group, the expression levels of copper basket protein, immunoglobulin IgG, immunoglobulin κ chain and complement C2 were significantly up-regulated. The expression levels of endothelial cell protein C receptor, human kallikrein-binding protein, pigment epithelium-derived factor, tetracycline, insulin-like growth factor 2, and thyroxine carrier protein were down-regulated. KEEG pathway analysis indicated that 10 kinds of proteins were closely related to Wnt signaling pathway, coagulation and complement cascades reaction signaling pathway and SLE signaling pathway. In summary, the differentially expressed proteins are obtained successfully in steroid-induced ONFH by proteomics, which provides theoretical basis for the diagnosis and pathogenesis of steroid-induced ONFH.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Lupus Erythematosus, Systemic, Femoral Head Necrosis, Proteomics, Tissue Engineering

CLC Number:

R446

Fan Siqi, Zeng Ping, Zhou Yi, Qin Gang, Liao Xiaobo, He Kaiyi. Screening differentially expressed proteins in the serum of patients with systemic lupus erythematosus combined with osteonecrosis of the femoral head by iTRAQ technology[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(3): 476-481.

Figures/Tables 2

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